ABSTRACT
Gene expression technology has made great progress with the continuous developments and researches of molecular biology. Though many systems to produce recombinant proteins have been studied, none of them is available so far to satisfy the needs completely. With the increasing demands of bioactive peptides and protein drugs, expression quantity and correct posttranslational folding and modifications are also needed under the circumstance which can make proteins more close to native conformation and higher activity and stability. Based on our previous work, we summarized the factors affecting the folding and modifications of recombinant proteins correctly from five aspects, including expression system and hosts, secretory expression, coexpression, fusion expression, and the culture conditions, as well as improvement strategies.
ABSTRACT
Purpose:To investigate the relationship between the expression tansforming growth factor ?(TGF ?) and the differentiation, invasion of colorectal adenocarcinoma.Methods:To observe the expression of transforming growth factor ? and transforming growth factor ? receptor in 68 samples of colorectal adenocarcinoma with immunohistochemical technique. Results:The cells with positive reaction of TGF ? antibody were scattered in the stroma. The cells with infiltrative growth had positive TGF ? staining.Conclusions:It was indicated that TGF ? might play an important role in the invasion and metastasis of colorectal adenocarcinoma.